A DIGE analysis of developing poplar leaves subjected to ozone reveals major changes in carbon metabolism.
Identifieur interne : 003C60 ( Main/Exploration ); précédent : 003C59; suivant : 003C61A DIGE analysis of developing poplar leaves subjected to ozone reveals major changes in carbon metabolism.
Auteurs : Sacha Bohler [Luxembourg (pays)] ; Matthieu Bagard ; Mouhssin Oufir ; Sébastien Planchon ; Lucien Hoffmann ; Yves Jolivet ; Jean-François Hausman ; Pierre Dizengremel ; Jenny RenautSource :
- Proteomics [ 1615-9853 ] ; 2007.
Descripteurs français
- KwdFr :
- Carbone (métabolisme), Données de séquences moléculaires (MeSH), Feuilles de plante (composition chimique), Feuilles de plante (effets des médicaments et des substances chimiques), Feuilles de plante (métabolisme), Oxydants photochimiques (pharmacologie), Ozone (pharmacologie), Pigments biologiques (analyse), Populus (anatomie et histologie), Populus (composition chimique), Populus (effets des médicaments et des substances chimiques), Protéines végétales (analyse), Protéomique (MeSH), Spectrométrie de masse MALDI (MeSH), Électrophorèse bidimensionnelle sur gel (méthodes).
- MESH :
- analyse : Pigments biologiques, Protéines végétales.
- anatomie et histologie : Populus.
- composition chimique : Feuilles de plante, Populus.
- effets des médicaments et des substances chimiques : Feuilles de plante, Populus.
- métabolisme : Carbone, Feuilles de plante.
- méthodes : Électrophorèse bidimensionnelle sur gel.
- pharmacologie : Oxydants photochimiques, Ozone.
- Données de séquences moléculaires, Protéomique, Spectrométrie de masse MALDI.
English descriptors
- KwdEn :
- Carbon (metabolism), Electrophoresis, Gel, Two-Dimensional (methods), Molecular Sequence Data (MeSH), Oxidants, Photochemical (pharmacology), Ozone (pharmacology), Pigments, Biological (analysis), Plant Leaves (chemistry), Plant Leaves (drug effects), Plant Leaves (metabolism), Plant Proteins (analysis), Populus (anatomy & histology), Populus (chemistry), Populus (drug effects), Proteomics (MeSH), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH).
- MESH :
- chemical , analysis : Pigments, Biological, Plant Proteins.
- chemical , metabolism : Carbon.
- anatomy & histology : Populus.
- chemistry : Plant Leaves, Populus.
- drug effects : Plant Leaves, Populus.
- metabolism : Plant Leaves.
- methods : Electrophoresis, Gel, Two-Dimensional.
- chemical , pharmacology : Oxidants, Photochemical, Ozone.
- Molecular Sequence Data, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Tropospheric ozone pollution is described as having major negative effects on plants, compromising plant survival. Carbon metabolism is especially affected. In the present work, the effects of chronic ozone exposure were evaluated at the proteomic level in developing leaves of young poplar plants exposed to 120 ppb of ozone for 35 days. Soluble proteins (excluding intrinsic membrane proteins) were extracted from leaves after 3, 14 and 35 days of ozone exposure, as well as 10 days after a recovery period. Proteins (pI 4 to 7) were analyzed by 2-D DIGE experiments, followed by MALDI-TOF-TOF identification. Additional observations were obtained on growth, lesion formation, and leaf pigments analysis. Although treated plants showed large necrotic spots and chlorosis in mature leaves, growth decreased only slightly and plant height was not affected. The number of abscised leaves was higher in treated plants, but new leaf formation was not affected. A decrease in chlorophylls and lutein contents was recorded. A large number of proteins involved in carbon metabolism were identified. In particular, proteins associated with the Calvin cycle and electron transport in the chloroplast were down-regulated. In contrast, proteins associated with glucose catabolism increased in response to ozone exposure. Other identified enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activity.
DOI: 10.1002/pmic.200600822
PubMed: 17486556
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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(analysis)</term><term>Plant Leaves (chemistry)</term><term>Plant Leaves (drug effects)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (analysis)</term><term>Populus (anatomy & histology)</term><term>Populus (chemistry)</term><term>Populus (drug effects)</term><term>Proteomics (MeSH)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Carbone (métabolisme)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (composition chimique)</term><term>Feuilles de plante (effets des médicaments et des substances chimiques)</term><term>Feuilles de plante (métabolisme)</term><term>Oxydants photochimiques (pharmacologie)</term><term>Ozone (pharmacologie)</term><term>Pigments biologiques (analyse)</term><term>Populus (anatomie et histologie)</term><term>Populus (composition chimique)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Protéines végétales (analyse)</term><term>Protéomique (MeSH)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Électrophorèse bidimensionnelle sur gel (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Pigments, Biological</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Carbon</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Pigments biologiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Plant Leaves</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" 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qualifier="pharmacology" xml:lang="en"><term>Oxidants, Photochemical</term><term>Ozone</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Molecular Sequence Data</term><term>Proteomics</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Données de séquences moléculaires</term><term>Protéomique</term><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Tropospheric ozone pollution is described as having major negative effects on plants, compromising plant survival. Carbon metabolism is especially affected. In the present work, the effects of chronic ozone exposure were evaluated at the proteomic level in developing leaves of young poplar plants exposed to 120 ppb of ozone for 35 days. Soluble proteins (excluding intrinsic membrane proteins) were extracted from leaves after 3, 14 and 35 days of ozone exposure, as well as 10 days after a recovery period. Proteins (pI 4 to 7) were analyzed by 2-D DIGE experiments, followed by MALDI-TOF-TOF identification. Additional observations were obtained on growth, lesion formation, and leaf pigments analysis. Although treated plants showed large necrotic spots and chlorosis in mature leaves, growth decreased only slightly and plant height was not affected. The number of abscised leaves was higher in treated plants, but new leaf formation was not affected. A decrease in chlorophylls and lutein contents was recorded. A large number of proteins involved in carbon metabolism were identified. In particular, proteins associated with the Calvin cycle and electron transport in the chloroplast were down-regulated. In contrast, proteins associated with glucose catabolism increased in response to ozone exposure. Other identified enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activity.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17486556</PMID><DateCompleted><Year>2007</Year><Month>07</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1615-9853</ISSN><JournalIssue CitedMedium="Print"><Volume>7</Volume><Issue>10</Issue><PubDate><Year>2007</Year><Month>May</Month></PubDate></JournalIssue><Title>Proteomics</Title><ISOAbbreviation>Proteomics</ISOAbbreviation></Journal><ArticleTitle>A DIGE analysis of developing poplar leaves subjected to ozone reveals major changes in carbon metabolism.</ArticleTitle><Pagination><MedlinePgn>1584-99</MedlinePgn></Pagination><Abstract><AbstractText>Tropospheric ozone pollution is described as having major negative effects on plants, compromising plant survival. Carbon metabolism is especially affected. In the present work, the effects of chronic ozone exposure were evaluated at the proteomic level in developing leaves of young poplar plants exposed to 120 ppb of ozone for 35 days. Soluble proteins (excluding intrinsic membrane proteins) were extracted from leaves after 3, 14 and 35 days of ozone exposure, as well as 10 days after a recovery period. Proteins (pI 4 to 7) were analyzed by 2-D DIGE experiments, followed by MALDI-TOF-TOF identification. Additional observations were obtained on growth, lesion formation, and leaf pigments analysis. Although treated plants showed large necrotic spots and chlorosis in mature leaves, growth decreased only slightly and plant height was not affected. The number of abscised leaves was higher in treated plants, but new leaf formation was not affected. A decrease in chlorophylls and lutein contents was recorded. A large number of proteins involved in carbon metabolism were identified. In particular, proteins associated with the Calvin cycle and electron transport in the chloroplast were down-regulated. In contrast, proteins associated with glucose catabolism increased in response to ozone exposure. Other identified enzymes are associated with protein folding, nitrogen metabolism and oxidoreductase activity.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Bohler</LastName><ForeName>Sacha</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre de Recherche Public--Gabriel Lippmann, Département Environnement et Agrobiotechnologies, Belvaux, Luxembourg.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bagard</LastName><ForeName>Matthieu</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Oufir</LastName><ForeName>Mouhssin</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Planchon</LastName><ForeName>Sébastien</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Hoffmann</LastName><ForeName>Lucien</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Jolivet</LastName><ForeName>Yves</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Hausman</LastName><ForeName>Jean-François</ForeName><Initials>JF</Initials></Author><Author ValidYN="Y"><LastName>Dizengremel</LastName><ForeName>Pierre</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Renaut</LastName><ForeName>Jenny</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Proteomics</MedlineTA><NlmUniqueID>101092707</NlmUniqueID><ISSNLinking>1615-9853</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010083">Oxidants, Photochemical</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010860">Pigments, Biological</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>66H7ZZK23N</RegistryNumber><NameOfSubstance UI="D010126">Ozone</NameOfSubstance></Chemical><Chemical><RegistryNumber>7440-44-0</RegistryNumber><NameOfSubstance UI="D002244">Carbon</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002244" MajorTopicYN="N">Carbon</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015180" MajorTopicYN="N">Electrophoresis, Gel, Two-Dimensional</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010083" MajorTopicYN="N">Oxidants, Photochemical</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010126" MajorTopicYN="N">Ozone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010860" MajorTopicYN="N">Pigments, Biological</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="Y">Plant Leaves</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="Y">Populus</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="N">anatomy & histology</QualifierName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D040901" MajorTopicYN="N">Proteomics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>5</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>7</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>5</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17486556</ArticleId><ArticleId IdType="doi">10.1002/pmic.200600822</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Luxembourg (pays)</li></country></list><tree><noCountry><name sortKey="Bagard, Matthieu" sort="Bagard, Matthieu" uniqKey="Bagard M" first="Matthieu" last="Bagard">Matthieu Bagard</name><name sortKey="Dizengremel, Pierre" sort="Dizengremel, Pierre" uniqKey="Dizengremel P" first="Pierre" last="Dizengremel">Pierre Dizengremel</name><name sortKey="Hausman, Jean Francois" sort="Hausman, Jean Francois" uniqKey="Hausman J" first="Jean-François" last="Hausman">Jean-François Hausman</name><name sortKey="Hoffmann, Lucien" sort="Hoffmann, Lucien" uniqKey="Hoffmann L" first="Lucien" last="Hoffmann">Lucien Hoffmann</name><name sortKey="Jolivet, Yves" sort="Jolivet, Yves" uniqKey="Jolivet Y" first="Yves" last="Jolivet">Yves Jolivet</name><name sortKey="Oufir, Mouhssin" sort="Oufir, Mouhssin" uniqKey="Oufir M" first="Mouhssin" last="Oufir">Mouhssin Oufir</name><name sortKey="Planchon, Sebastien" sort="Planchon, Sebastien" uniqKey="Planchon S" first="Sébastien" last="Planchon">Sébastien Planchon</name><name sortKey="Renaut, Jenny" sort="Renaut, Jenny" uniqKey="Renaut J" first="Jenny" last="Renaut">Jenny Renaut</name></noCountry><country name="Luxembourg (pays)"><noRegion><name sortKey="Bohler, Sacha" sort="Bohler, Sacha" uniqKey="Bohler S" first="Sacha" last="Bohler">Sacha Bohler</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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